Effect of Pre-sleep Casein and Tryptophan Supplementation on Energy Expenditure Before, During, and After Exercise in Active Females

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Hannah Nelson
Silvio P. Valladão
Neil Schwarz
Melinda Valliant
Thomas L. Andre


Protein supplementation, Metabolic rate, Perceived sleep


Introduction: The purpose of this study was to determine the impact of pre-sleep casein protein and tryptophan supplement (SUP) ingestion on sleep quality and morning energy expenditure prior to, during, and post-exercise. Methods: Thirteen aerobically active females (age = 22.6 ± 1.9yrs; ht = 1.65 ± 0.06m; wt = 60.5 ± 9.6kg; %bf = 22.5 ± 4.3) ingested SUP or placebo (PLA) 30 min prior to going to bed in 2 trials. The following morning, perceived sleep quality and satiety were assessed, and resting energy expenditure (REE) was measured for 30 minutes before and after 20 min of exercise at 40-50% of VO2max while measuring exercise energy expenditure (EEE). Results: Sleep quality and satiety during SUP was not significantly different than PLA trial (61.6 ± 21.5 vs 68.4 ± 20.5 mm; p = 0.39; 29.6 ± 8.2 vs 23.9 ± 6.6 mm; p = 0.43). REE before and after exercise during SUP and PLA were not significantly different F(1,12)=0,141, p = 0.71. EEE during SUP was not significantly different than PLA (112.1 ± 20.4 vs 112.2 ± 21.8 kcals; p = 0.91). Conclusion: No changes were seen when ingesting SUP prior to sleep on morning EEE and REE before and after exercise.

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