Attenuation of Delayed Onset Muscle Soreness with Acute Consumption of Essential Amino Acids Original Research
Main Article Content
Keywords
Amino Acid Supplementation, Anaerobic Exercise, Aerobic Exercise
Abstract
Introduction: Prior studies of the acute benefits of protein supplementation have determined a benefit in improving post-exercise muscle anabolism and aiding the recovery of muscle function and performance. Previous acute protein supplement studies in post-exercise protein synthesis and anabolic intracellular signaling reported no attenuation in muscle damage or elevated muscle function. The aim of this study is to implement a specific content of essential amino acids with resistance and aerobic exercises to quantify the difference in strength, endurance, and flexibility during the delayed onset muscle soreness common with a new exercise protocol.
Methods: We enrolled 42 participants (22 EAA and 20 Controls) completed an hour-long aerobic and resistance exercise protocol including flexibility, resistance, and aerobic exercises for three consecutive days. The study participants were randomly assigned to the EAA (6.6g) per day (EAA + Gatorade) group or the control (Gatorade) group. The data was analyzed in a double-blinded format.
Results: Both groups improved the initial flexibility respectively throughout the three exercise days but were not significantly different (p=0.32) in the sit and reach. For the resistance/power activities, the EAA group improved in the repetitions for push-ups (p=0.014 vs 0.21) and dips (0.0002 vs 0.59) compared to the controls. The EAA group was faster although not statistically significant in the 20-meter sprint and improved in the 1.5-mile run during the third day (P=0.002 vs 0.48) compared to the control group.
Conclusions: The data in the results supports that acute ingestion of the essential amino acid supplements provides increased physical performance and decreases the DOMS symptoms in sedentary participants over the three-day trial period of exercise.
References
2. Churchward-Venne TA, Burd NA, Phillips SM. Nutritional regulation of muscle protein synthesis with resistance exercise: strategies to enhance anabolism. Nutr Metab (Lond). 2012;9(1):40. Published 2012 May 17. doi:10.1186/1743-7075-9-40
3. Churchward-Venne TA, Breen L, Di Donato DM, et al. Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr. 2014;99(2):276-286. doi:10.3945/ajcn.113.068775
4. Clarkson PM, Nosaka K, Braun B. Muscle function after exercise-induced muscle damage and rapid adaptation. Med Sci Sports Exerc. 1992;24(5):512-520.
5. Clarkson PM, Hubal MJ. Exercise-induced muscle damage in humans. Am J Phys Med Rehabil. 2002;81(11 Suppl):S52-S69. doi:10.1097/00002060-200211001-00007
6. Coombes JS, McNaughton LR. Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise. J Sports Med Phys Fitness. 2000;40(3):240-246.
7. Hohenauer E, Clarys P, Baeyens JP, Clijsen R. Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol. J Vis Exp. 2017;(124):55612. Published 2017 Jun 8. doi:10.3791/55612
8. Howatson G, Hoad M, Goodall S, Tallent J, Bell PG, French DN. Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr. 2012;9:20. Published 2012 Jul 12. doi:10.1186/1550-2783-9-20
9. Jackman SR, Witard OC, Jeukendrup AE, Tipton KD. Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Med Sci Sports Exerc. 2010;42(5):962-970. doi:10.1249/MSS.0b013e3181c1b798
10. Kephart WC, Mumford PW, McCloskey AE, et al. Post-exercise branched chain amino acid supplementation does not affect recovery markers following three consecutive high intensity resistance training bouts compared to carbohydrate supplementation. J Int Soc Sports Nutr. 2016;13:30. Published 2016 Jul 26. doi:10.1186/s12970-016-0142-y
11. Levenhagen DK, Carr C, Carlson MG, Maron DJ, Borel MJ, Flakoll PJ. Postexercise protein intake enhances whole-body and leg protein accretion in humans. Med Sci Sports Exerc. 2002;34(5):828-837. doi:10.1097/00005768-200205000-00016
12. Lim IS. Effects of branched-chain amino acid supplement on knee peak torque and indicators of muscle damage following isokinetic exercise-induced delayed onset muscle soreness. Phys Act Nutr. 2020;24(4):28-33. doi: 10.20463/pan.2020.0025.
13. Millard-Stafford M, Warren GL, Thomas LM, Doyle JA, Snow T, Hitchcock K. Recovery from run training: efficacy of a carbohydrate-protein beverage? [published correction appears in Int J Sport Nutr Exerc Metab. 2006 Feb;16(1):3 p following 125]. Int J Sport Nutr Exerc Metab. 2005;15(6):610-624. doi:10.1123/ijsnem.15.6.610
14. Nosaka K, Sacco P, Mawatari K. Effects of amino acid supplementation on muscle soreness and damage. Int J Sport Nutr Exerc Metab. 2006;16(6):620-635. doi:10.1123/ijsnem.16.6.620
15. Paddon-Jones D, Børsheim E, Wolfe RR. Potential ergogenic effects of arginine and creatine supplementation. J Nutr. 2004;134(10 Suppl):2888S-2895S. doi:10.1093/jn/134.10.2888s
16. Pasiakos SM, Lieberman HR, McLellan TM. Effects of protein supplements on muscle damage, soreness and recovery of muscle function and physical performance: a systematic review. Sports Med. 2014;44(5):655-670. doi:10.1007/s40279-013-0137-7
17. Peake JM, Neubauer O, Della Gatta PA, Nosaka K. Muscle damage and inflammation during recovery from exercise. J Appl Physiol (1985). 2017;122(3):559-570. doi:10.1152/japplphysiol.00971.2016
18. Proske U, Morgan DL. Muscle damage from eccentric exercise: mechanism, mechanical signs, adaptation, and clinical applications. J Physiol. 2001;537(Pt 2):333-345. doi:10.1111/j.1469-7793.2001.00333.x
19. Ra SG, Miyazaki T, Kojima R, et al. Effect of BCAA supplement timing on exercise-induced muscle soreness and damage: a pilot placebo-controlled double-blind study. J Sports Med Phys Fitness. 2018;58(11):1582-1591. doi:10.23736/S0022-4707.17.07638-1
20. Romano-Ely BC, Todd MK, Saunders MJ, Laurent TS. Effect of an isocaloric carbohydrate-protein-antioxidant drink on cycling performance. Med Sci Sports Exerc. 2006;38(9):1608-1616. doi:10.1249/01.mss.0000229458.11452.e9
21. Rowlands DS, Thorp RM, Rossler K, Graham DF, Rockell MJ. Effect of protein-rich feeding on recovery after intense exercise. Int J Sport Nutr Exerc Metab. 2007;17(6):521-543. doi:10.1123/ijsnem.17.6.521
22. Saunders MJ, Kane MD, Todd MK. Effects of a carbohydrate-protein beverage on cycling endurance and muscle damage. Med Sci Sports Exerc. 2004;36(7):1233-1238. doi:10.1249/01.mss.0000132377.66177.9f
23. Valentine RJ, Saunders MJ, Todd MK, St Laurent TG. Influence of carbohydrate-protein beverage on cycling endurance and indices of muscle disruption. Int J Sport Nutr Exerc Metab. 2008;18(4):363-378. doi:10.1123/ijsnem.18.4.363
24. Wilburn DT, Machek SB, Cardaci TD, Willoughby DS. Carbohydrate-Induced Insulin Signaling Activates Focal Adhesion Kinase: A Nutrient and Mechanotransduction Crossroads. Nutrients. 2020;12(10):3145. Published 2020 Oct 15. doi:10.3390/nu12103145
25. Willoughby DS, Stout JR, Wilborn CD. Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength. Amino Acids. 2007;32(4):467-477. doi:10.1007/s00726-006-0398-7
26. Wolfe RR. Branched-chain amino acids and muscle protein synthesis in humans: myth or reality?. J Int Soc Sports Nutr. 2017;14:30. Published 2017 Aug 22. doi:10.1186/s12970-017-0184-9