The Effects of a Multi-Ingredient Dietary Supplement on Recovery from Delayed-Onset Muscle Soreness Original Research

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Cassandra Evans
Jason Curtis
Flavia Pereira
Jose Rojas
Maria Berrocales
Kristiina Kinnunen
Antonio Crisanti
Kendall Andries
Leilani Batista
Juan Carlos Santana
Lia Jiannine
Jaime Tartar
Jose Antonio


Pain, Exercise, Nutrition


Introduction: The purpose of this investigation was to determine the effects of multi-ingredient dietary supplement on indices of muscle recovery on delayed-onset muscle soreness (DOMS).

Methods: In a randomized, placebo-controlled trial, healthy exercise-trained subjects (n=24) consumed the treatment (i.e., Caraflame®: Retinyl Palmitate (Vit. A) 3.3 mg, Sodium Butyrate 175 mg, and Beta-Caryophyllene 30 mg or placebo (i.e., Maltodextrin 1000mg) daily over a 14-day period. Subjects completed the DOMS protocol and were assessed for changes in pain (visual analog scale (VAS) and a pressure algometer), strength (1-RM), and inflammatory markers (Interleukin-1b, Interleukin-6 and C-reactive protein). A dependent samples t-test was used to determine differences between groups with regard to the delta score. A p-value of P<0.05 was used to determine significance.

Results: All subjects were physically active, healthy adults (Mean±SD – Age 23.5±7, Height 170±12.7 cm, Body Mass 71.0±19.57 kg, % body fat 24.3±10.6). A statistically significant difference was found for the assessment of pain threshold via VAS. Subjects in the treatment group exhibited a higher pain threshold two days post-DOMS (i.e., delta score data). No significant differences between groups for arm circumference, 1-RM, pain assessed by algometer, or arm circumference between the groups. Furthermore, there were no significant differences between groups for inflammatory markers (CRP, IL-6, and IL-1b). 

Conclusions: Based on this preliminary investigation, two weeks of a multi-ingredient dietary supplement may decrease the subjective perception of delayed-onset muscle soreness in exercise-trained adults.

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