The Effects of Probiotic Supplementation in Active Men and Women

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Jose Antonio
Alex Leaf
Cassandra Carson
Anya Ellerbroek
Cara Axelrod
Tobin Silver
Victoria Burgess
Corey Peacock


body composition, gut, microbiome, prebiotic, fat mass, obesity, abdominal fat


Introduction: There is evidence in rodents as well as obese adults that probiotic supplementation can promote a decrease in fat mass. For instance, Bifidobacterium animalis ssp. lactis 420 (B420) has been shown to decrease abdominal fat mass. Therefore, our laboratory determined the effects of probiotic supplementation on body composition in a group of active men and women in a double-blind, placebo-controlled two- arm investigation.
Methods: Twenty subjects participated in this investigation (6 male, 14 female). All were actively participating in aerobic and/or resistance training for a period of at least one year. Subjects were randomly assigned to a group that received either a placebo (maltodextrin) or an encapsulated probiotic (one capsule) containing 5 billion Bifidobacterium BR03 and 5 billion Streptococcus thermophilus FP4 (Probiotical, Novara, Italy). Subjects consumed one capsule daily during the 6-week treatment period. Furthermore, subjects were instructed to not alter their diet or training regimen during this time. Body composition was assessed via dual-energy x-ray absorptiometry (DXA) (Hologic Horizon W, Danbury CT USA). Data are presented as the mean ± SD. An ANOVA was used to assess differences between groups.
Results: The physical characteristics of the placebo and probiotic groups were as follows: Placebo – Age 25±4 years, Height 168±7 centimeters; Probiotic – Age 30±8 years, Height 166±8 centimeters. Six weeks of probiotic supplementation had no effect (p > 0.05 for all) on body weight, lean body mass, fat mass, bone mineral content, body fat percentage or trunk fat mass.
Conclusions: Six weeks of daily supplementation with a probiotic containing 5 billion Bifidobacterium BR03 and 5 billion Streptococcus thermophilus FP4 in active men and women has no effect on body composition.

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