Inositol-Stabilized Arginine Silicate Reduces Exercise Induced Muscle Damage and Increases Perceived Energy Original Research

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Danielle Greenberg
Katie Emerson
Sarah Ojalvo Perez
Sarah Sylla
James Komorowski


Nitric Oxide, hyperemia, myoglobin, salivary nitrite


Introduction: Inositol-stabilized arginine silicate (ASI; Nitrosigine®) significantly increases circulating arginine and nitric oxide (NO).  We examined ASI effects on objective and subjective indicators of muscle recovery, blood flow and energy. 

Methods:  In a double-blind, placebo-controlled crossover-design, subjects (n=16) were given ASI (1,500 mg/day) or placebo for 4 days, with a 7-day washout period. Measurements occurred at baseline, 24, 48, and 72 h. On test days, subjects performed stress inducing leg extension exercises associated with muscle soreness. Following exercise, recovery markers creatine kinase (CK), myoglobin and lactate dehydrogenase (LDH), doppler ultrasound blood flow, leg circumference, salivary nitrite tests were measured. The Profile Mood States (POMS), VAS scales, vigor-activity cognitive tests were administered.    

Results: Serum CK but not LDH was significantly reduced in the ASI group on day 1 and 24, 48, and 72 h post-exercise (p<0.05); myoglobin was reduced on d1 and at 24 h post-exercise. No negative heart rate or blood pressure effects were observed. Reactive hyperemia indicated by leg circumference showed greater increases in the ASI group at 72 h (p<0.05).  No differences were found in salivary nitrite levels (p=0.265).  Perceived energy POMS responses increased in the ASI group compared to placebo (p=0.039) but no differences were found in subjective muscle recovery as determined by VASs.

Conclusions: ASI may be beneficial for fitness goals by increasing blood flow, and reducing muscle damage and perceived energy.

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